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KMID : 0351819980390010023
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Kyunpook University Medical Journal
1998 Volume.39 No. 1 p.23 ~ p.32
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Mode of Human B type Hepatitis Virus Infection on Primary Human Adult and Fetal Hepatocytes
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Choi Won-Man
Yun Young-Kook
Chung Tai-Ho
Shim Jae-Koung
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Abstract
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Considerable progress has been made ir. the immune prophylaxis of hepatitis B vitus(HBV) infection and in the understanding of its replication and pathogenecir: at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacling, and the zany steps of viral life cycle are now vet well understood. Primary human hepatocytes are reported to be susceptible to HBV10~1´, but these systems are not practical because they are shoe-lived and require primary expaanted human liver. However, it is uncertain vet whether primary hepatoc;nes of human fetal liver are susceptible to HBV for infection or not.
we have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy or adult primary hepatocytes. To investigate the infectivity of HBV for adult and fetal hepatoc;nes, RT-PCR was performed. Total 12NAs were isolated from hepatocvtes harvested at different times after infection process and then use for cDNA synthesis and PCR. HBV DNA was not detected in uninfected and infected fetal hepatocytes_ However, HBV DNA was detected in infected adult heparocytes. The cloned HBV DNA(pHBV 315) and RNA from serum carried hieh level of HBV DNA ,,yore used as positive controls. From these data showed the HBV inr´ec:ivity to the adult ite~atocvte ,vas siartiticantl~~ hi~~her than the one to the fetal hepatoc;ne. above -exult may prove :he chronicity of B type viral hepatitis in men and existance or´ so called healthy carrier.
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